Genotyping and primer details


Genotyping and primer details for 566H08 - At2g44090
Line ID 566H08
Locus At2g44090 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:18239898 of line 566H08 Chr2:18239898
   
Insertion allele amplicon 8474 - 47jd
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 47jd: 5'-CTTCTTGTGTTTCATCTTGCCATA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:18240395
   
wt amplicon (over InsPos) 05jz - 47jd
wt amplicon start Chr2:18239426
wt amplicon end Chr2:18240395
wt amplicon size 970 bp
Locus specific primer 47jd: 5'-CTTCTTGTGTTTCATCTTGCCATA-3'
2nd locus specific primer 05jz: 5'-TTTACTATAATGCGAGGCAAAACC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11