Genotyping and primer details


Genotyping and primer details for 567B06 - At1g19110
Line ID 567B06
Locus At1g19110 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:6604756 of line 567B06 Chr1:6604756
   
Insertion allele amplicon 8474 - 50by
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 50by: 5'-AAAATGAAGAAGCAAATCCTGAAA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:6605169
   
wt amplicon (over InsPos) 03dt - 50by
wt amplicon start Chr1:6604333
wt amplicon end Chr1:6605169
wt amplicon size 837 bp
Locus specific primer 50by: 5'-AAAATGAAGAAGCAAATCCTGAAA-3'
2nd locus specific primer 03dt: 5'-ATAACCCGGATCGTTTTATTTACC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11