Genotyping and primer details


Genotyping and primer details for 567D02 - At5g41080
Line ID 567D02
Locus At5g41080 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:16443106 of line 567D02 Chr5:16443106
   
Insertion allele amplicon 8474 - 25fp
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 25fp: 5'-AATCTACAATCACTCCATCGATCC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:16443662
   
wt amplicon (over InsPos) 09gf - 25fp
wt amplicon start Chr5:16442793
wt amplicon end Chr5:16443662
wt amplicon size 870 bp
Locus specific primer 25fp: 5'-AATCTACAATCACTCCATCGATCC-3'
2nd locus specific primer 09gf: 5'-ATCAGACCGTCTATGAACGAGAGT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11