Genotyping and primer details


Genotyping and primer details for 572B09 - At1g01830
Line ID 572B09
Locus At1g01830 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:299826 of line 572B09 Chr1:299826
   
Insertion allele amplicon 8474 - 29ny
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 29ny: 5'-AGTTTACAGGAAGATGGAAGACGA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:300278
   
wt amplicon (over InsPos) 43si - 29ny
wt amplicon start Chr1:299379
wt amplicon end Chr1:300278
wt amplicon size 900 bp
Locus specific primer 29ny: 5'-AGTTTACAGGAAGATGGAAGACGA-3'
2nd locus specific primer 43si: 5'-CCATGTGTTCTCTTGATCCTAACA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11