Genotyping and primer details


Genotyping and primer details for 585B06 - At2g26610
Line ID 585B06
Locus At2g26610 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:11321025 of line 585B06 Chr2:11321025
   
Insertion allele amplicon 8474 - NU68
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer NU68: 5'-TGAACACTAGAAATGTTGCTTTGG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:11320660
   
wt amplicon (over InsPos) 27si - NU68
wt amplicon start Chr2:11320660
wt amplicon end Chr2:11321363
wt amplicon size 704 bp
Locus specific primer NU68: 5'-TGAACACTAGAAATGTTGCTTTGG-3'
2nd locus specific primer 27si: 5'-AAATACGCCAAGTGAACCATTTAC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11