Genotyping and primer details


Genotyping and primer details for 589A02 - At1g63250
Line ID 589A02
Locus At1g63250 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:23466139 of line 589A02 Chr1:23466139
   
Insertion allele amplicon 8474 - 19wm
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 19wm: 5'-ATCAAATTCCTTTACACAGCCAGT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:23465433
   
wt amplicon (over InsPos) 80ww - 19wm
wt amplicon start Chr1:23465433
wt amplicon end Chr1:23466959
wt amplicon size 1527 bp
Locus specific primer 19wm: 5'-ATCAAATTCCTTTACACAGCCAGT-3'
2nd locus specific primer 80ww: 5'-TTAAAGAAAACACTTCACCGGATT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11