Genotyping and primer details


Genotyping and primer details for 597G07 - At5g26120
Line ID 597G07
Locus At5g26120 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:9124185 of line 597G07 Chr5:9124185
   
Insertion allele amplicon 8474 - FV34
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer FV34: 5'-TATGTTGTTACAGACCGTTCTTCG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:9124767
   
wt amplicon (over InsPos) 07id - FV34
wt amplicon start Chr5:9123825
wt amplicon end Chr5:9124767
wt amplicon size 943 bp
Locus specific primer FV34: 5'-TATGTTGTTACAGACCGTTCTTCG-3'
2nd locus specific primer 07id: 5'-CATCATCAGTCCAGTACTTCCAAA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11