Genotyping and primer details


Genotyping and primer details for 603A06 - At5g09930
Line ID 603A06
Locus At5g09930 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:3098407 of line 603A06 Chr5:3098407
   
Insertion allele amplicon 8474 - BA28
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer BA28: 5'-TTGATCTGTATTTCTGATGCAAGC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:3098775
   
Insertion allele amplicon 3144 - BA29
T-DNA primer 3144: 5'-GTGGATTGATGTGATATCTCC-3'
Locus specific primer BA29: 5'-GCATAGTCCATTAAACCACCATCT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:3097958
   
wt amplicon (over InsPos) BA28 - BA29
wt amplicon start Chr5:3097958
wt amplicon end Chr5:3098775
wt amplicon size 818 bp
Locus specific primer BA29: 5'-GCATAGTCCATTAAACCACCATCT-3'
2nd locus specific primer BA28: 5'-TTGATCTGTATTTCTGATGCAAGC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11