Genotyping and primer details


Genotyping and primer details for 605F08 - At3g53390
Line ID 605F08
Locus At3g53390 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:19793517 of line 605F08 Chr3:19793517
   
Insertion allele amplicon 8474 - 29et
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 29et: 5'-CATGGCAAAACTGTTACTCAGGTA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:19792943
   
wt amplicon (over InsPos) 34go - 29et
wt amplicon start Chr3:19792943
wt amplicon end Chr3:19793825
wt amplicon size 883 bp
Locus specific primer 29et: 5'-CATGGCAAAACTGTTACTCAGGTA-3'
2nd locus specific primer 34go: 5'-TCCTGTAACTGCTGTCTCAGTGAT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11