Genotyping and primer details


Genotyping and primer details for 607A07 - At4g31250
Line ID 607A07
Locus At4g31250 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:15180609 of line 607A07 Chr4:15180609
   
Insertion allele amplicon 3144 - 68wn
T-DNA primer 3144: 5'-GTGGATTGATGTGATATCTCC-3'
Locus specific primer 68wn: 5'-CAACAAAACTCATCAGATTCCTCA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:15179889
   
Insertion allele amplicon 8474 - EU19
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer EU19: 5'-CTGAAGGTTCATCTTGAAGGAAAC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:15181301
   
wt amplicon (over InsPos) ST05 - EU19
wt amplicon start Chr4:15180378
wt amplicon end Chr4:15181301
wt amplicon size 924 bp
Locus specific primer EU19: 5'-CTGAAGGTTCATCTTGAAGGAAAC-3'
2nd locus specific primer ST05: 5'-TTTCTGTAGACCTTGGAGTCCTGT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11