Genotyping and primer details


Genotyping and primer details for 608F01 - At1g67090
Line ID 608F01
Locus At1g67090 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:25049115 of line 608F01 Chr1:25049115
   
Insertion allele amplicon 3144 - FP94
T-DNA primer 3144: 5'-GTGGATTGATGTGATATCTCC-3'
Locus specific primer FP94: 5'-TAAGGGTCTCAACACCTCTCCTTA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:25049477
   
Insertion allele amplicon 8474 - ND03
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer ND03: 5'-AAACCACATGTTACAAACCACTCA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:25048708
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11