Genotyping and primer details


Genotyping and primer details for 611F01 - At3g59360
Line ID 611F01
Locus At3g59360 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:21939589 of line 611F01 Chr3:21939589
   
Insertion allele amplicon 8474 - MP50
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer MP50: 5'-ACCTGGATTAAAAGCATCACAATC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:21939236
   
wt amplicon (over InsPos) 05sl - MP50
wt amplicon start Chr3:21939236
wt amplicon end Chr3:21939865
wt amplicon size 630 bp
Locus specific primer MP50: 5'-ACCTGGATTAAAAGCATCACAATC-3'
2nd locus specific primer 05sl: 5'-TCTTTTCTTTTTCGCTCATTGTTT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11