Genotyping and primer details


Genotyping and primer details for 625G07 - At1g55510
Line ID 625G07
Locus At1g55510 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:20723742 of line 625G07 Chr1:20723742
   
Insertion allele amplicon 8474 - 12ht
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 12ht: 5'-AGAATCTGTTTCTTTCTTCACCCA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:20723337
   
wt amplicon (over InsPos) 01io - 12ht
wt amplicon start Chr1:20723337
wt amplicon end Chr1:20724129
wt amplicon size 793 bp
Locus specific primer 12ht: 5'-AGAATCTGTTTCTTTCTTCACCCA-3'
2nd locus specific primer 01io: 5'-TCTCTACAATTGCTCGATTACCCT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11