Genotyping and primer details


Genotyping and primer details for 630C04 - At5g37450
Line ID 630C04
Locus At5g37450 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:14853932 of line 630C04 Chr5:14853932
   
Insertion allele amplicon 8474 - EU24
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer EU24: 5'-TTGCTTTCTTCCTTGTATTGTCCT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:14854263
   
wt amplicon (over InsPos) SP06 - EU24
wt amplicon start Chr5:14853338
wt amplicon end Chr5:14854263
wt amplicon size 926 bp
Locus specific primer EU24: 5'-TTGCTTTCTTCCTTGTATTGTCCT-3'
2nd locus specific primer SP06: 5'-TGTTAAAGCAACATACAGGAGTGC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11