Genotyping and primer details


Genotyping and primer details for 630G07 - At2g34920
Line ID 630G07
Locus At2g34920 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:14729875 of line 630G07 Chr2:14729875
   
Insertion allele amplicon 8474 - 30vh
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 30vh: 5'-TCCGAGGCCTAGATATTTCTGTAA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:14729310
   
wt amplicon (over InsPos) 82vo - 30vh
wt amplicon start Chr2:14729310
wt amplicon end Chr2:14730060
wt amplicon size 751 bp
Locus specific primer 30vh: 5'-TCCGAGGCCTAGATATTTCTGTAA-3'
2nd locus specific primer 82vo: 5'-TTAACAAACGATCCAGTTTCAGTG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11