Genotyping and primer details


Genotyping and primer details for 638B11 - At5g58900
Line ID 638B11
Locus At5g58900 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:23784193 of line 638B11 Chr5:23784193
   
Insertion allele amplicon 8474 - 51hw
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 51hw: 5'-AAAGTTCCGAGATATGTTTCTCCA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:23783632
   
wt amplicon (over InsPos) 27ix - 51hw
wt amplicon start Chr5:23783632
wt amplicon end Chr5:23784692
wt amplicon size 1061 bp
Locus specific primer 51hw: 5'-AAAGTTCCGAGATATGTTTCTCCA-3'
2nd locus specific primer 27ix: 5'-CAGAGCTAGGTTTTAGTTTTCGGA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11