Genotyping and primer details


Genotyping and primer details for 644G01 - At3g15530
Line ID 644G01
Locus At3g15530 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:5253641 of line 644G01 Chr3:5253641
   
Insertion allele amplicon 8474 - 50fr
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 50fr: 5'-TAGTGTCGAACCTCCAGACTTCTT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:5253164
   
wt amplicon (over InsPos) 01gv - 50fr
wt amplicon start Chr3:5253164
wt amplicon end Chr3:5254249
wt amplicon size 1086 bp
Locus specific primer 50fr: 5'-TAGTGTCGAACCTCCAGACTTCTT-3'
2nd locus specific primer 01gv: 5'-ACTCGTAGATCGGATTTTTGTTTG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11