Genotyping and primer details


Genotyping and primer details for 645A11 - At1g59740
Line ID 645A11
Locus At1g59740 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:21969903 of line 645A11 Chr1:21969903
   
Insertion allele amplicon 8474 - 25kq
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 25kq: 5'-ACATAATCGGGATTTGTCAATGAT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:21970351
   
wt amplicon (over InsPos) 08li - 25kq
wt amplicon start Chr1:21969500
wt amplicon end Chr1:21970351
wt amplicon size 852 bp
Locus specific primer 25kq: 5'-ACATAATCGGGATTTGTCAATGAT-3'
2nd locus specific primer 08li: 5'-GATGACTATGGGCATCATTAGCTT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11