Genotyping and primer details


Genotyping and primer details for 645E09 - At5g65440
Line ID 645E09
Locus At5g65440 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:26154143 of line 645E09 Chr5:26154143
   
Insertion allele amplicon 8474 - 09hd
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 09hd: 5'-GGGGTTAATACATCGTTGAGAAGA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:26154642
   
wt amplicon (over InsPos) 07ig - 09hd
wt amplicon start Chr5:26153800
wt amplicon end Chr5:26154642
wt amplicon size 843 bp
Locus specific primer 09hd: 5'-GGGGTTAATACATCGTTGAGAAGA-3'
2nd locus specific primer 07ig: 5'-GATTCTCTCTCTCTCACTCGCATT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11