Genotyping and primer details


Genotyping and primer details for 648D10 - At5g47820
Line ID 648D10
Locus At5g47820 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:19367241 of line 648D10 Chr5:19367241
   
Insertion allele amplicon 8474 - 14eb
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 14eb: 5'-TCTTCTTCTTCTTCCAGTTTCGTT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:19366645
   
wt amplicon (over InsPos) 01eh - 14eb
wt amplicon start Chr5:19366645
wt amplicon end Chr5:19367803
wt amplicon size 1159 bp
Locus specific primer 14eb: 5'-TCTTCTTCTTCTTCCAGTTTCGTT-3'
2nd locus specific primer 01eh: 5'-AATATCACAAGTCACCTGGACTGA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11