Genotyping and primer details


Genotyping and primer details for 649C02 - At3g11330
Line ID 649C02
Locus At3g11330 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:3554749 of line 649C02 Chr3:3554749
   
Insertion allele amplicon 8474 - 35fm
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 35fm: 5'-TATCCCAACAACCTCCTCATTAAC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:3554123
   
wt amplicon (over InsPos) 14ho - 35fm
wt amplicon start Chr3:3554123
wt amplicon end Chr3:3555315
wt amplicon size 1193 bp
Locus specific primer 35fm: 5'-TATCCCAACAACCTCCTCATTAAC-3'
2nd locus specific primer 14ho: 5'-TATCCCGAGTTCAATCATCTTTTC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11