Genotyping and primer details


Genotyping and primer details for 650F02 - At1g21680
Line ID 650F02
Locus At1g21680 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:7613850 of line 650F02 Chr1:7613850
   
Insertion allele amplicon 8474 - CQ54
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer CQ54: 5'-CATCTCATATACGTCACCGAGAGA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:7613334
   
wt amplicon (over InsPos) 09ah - CQ54
wt amplicon start Chr1:7613334
wt amplicon end Chr1:7614466
wt amplicon size 1133 bp
Locus specific primer CQ54: 5'-CATCTCATATACGTCACCGAGAGA-3'
2nd locus specific primer 09ah: 5'-TCAACCTCTGTTCTCTCTGTAGCA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11