Genotyping and primer details


Genotyping and primer details for 655E04 - At3g25780
Line ID 655E04
Locus At3g25780 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:9410229 of line 655E04 Chr3:9410229
   
Insertion allele amplicon 8474 - 23eq
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 23eq: 5'-AACGAAGGAGATAGAAACAGTCCA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:9409912
   
wt amplicon (over InsPos) 07fl - 23eq
wt amplicon start Chr3:9409912
wt amplicon end Chr3:9410832
wt amplicon size 921 bp
Locus specific primer 23eq: 5'-AACGAAGGAGATAGAAACAGTCCA-3'
2nd locus specific primer 07fl: 5'-GATAGCCAGCAATAACTAGGTTGC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11