Genotyping and primer details


Genotyping and primer details for 658E04 - At5g13560
Line ID 658E04
Locus At5g13560 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:4362069 of line 658E04 Chr5:4362069
   
Insertion allele amplicon 8474 - 35uj
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 35uj: 5'-CAGATCAGGGAGACAATCAGGTAT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:4362344
   
wt amplicon (over InsPos) 33vc - 35uj
wt amplicon start Chr5:4361581
wt amplicon end Chr5:4362344
wt amplicon size 764 bp
Locus specific primer 35uj: 5'-CAGATCAGGGAGACAATCAGGTAT-3'
2nd locus specific primer 33vc: 5'-ACTGCAGTTCTTTCTCCAGTTCAT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11