Genotyping and primer details


Genotyping and primer details for 658H04 - At2g14510
Line ID 658H04
Locus At2g14510 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:6173181 of line 658H04 Chr2:6173181
   
Insertion allele amplicon 8474 - EU34
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer EU34: 5'-AACCAACACGATTATAGTAACGGC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:6172766
   
wt amplicon (over InsPos) SP08 - EU34
wt amplicon start Chr2:6172766
wt amplicon end Chr2:6173667
wt amplicon size 902 bp
Locus specific primer EU34: 5'-AACCAACACGATTATAGTAACGGC-3'
2nd locus specific primer SP08: 5'-GTCTACGGCTTAAGAGGATGTACG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11