Genotyping and primer details


Genotyping and primer details for 660A09 - At5g63280
Line ID 660A09
Locus At5g63280 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:25368225 of line 660A09 Chr5:25368225
   
Insertion allele amplicon CR3S - CB35
T-DNA primer CR3S: 5'-TTGAGCATATAAGAAACCCTTAGT-3'
Locus specific primer CB35: 5'-TGCAAGAAAAGTTTCAATGATGAG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:25367794
   
Insertion allele amplicon 8474 - NL21
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer NL21: 5'-CCATCTTATGACGGTTTTGTTTTT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:25368853
   
wt amplicon (over InsPos) CB44 - NL21
wt amplicon start Chr5:25367794
wt amplicon end Chr5:25368853
wt amplicon size 1060 bp
Locus specific primer NL21: 5'-CCATCTTATGACGGTTTTGTTTTT-3'
2nd locus specific primer CB44: 5'-TGCAAGAAAAGTTTCAATGATGAG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11