Genotyping and primer details


Genotyping and primer details for 666A09 - At3g12290
Line ID 666A09
Locus At3g12290 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:3920867 of line 666A09 Chr3:3920867
   
Insertion allele amplicon 8474 - 52hw
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 52hw: 5'-TCGTCAAGCCAAGTACACAATTAC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:3921396
   
wt amplicon (over InsPos) 05jm - 52hw
wt amplicon start Chr3:3920533
wt amplicon end Chr3:3921396
wt amplicon size 864 bp
Locus specific primer 52hw: 5'-TCGTCAAGCCAAGTACACAATTAC-3'
2nd locus specific primer 05jm: 5'-CCGAAACATATTAATGAGGAGCAT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11