Genotyping and primer details


Genotyping and primer details for 666E12 - At3g14390
Line ID 666E12
Locus At3g14390 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:4808936 of line 666E12 Chr3:4808936
   
Insertion allele amplicon 8474 - 43sj
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 43sj: 5'-CAAACTTGTGAACAAAAAGCTTGA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:4809254
   
wt amplicon (over InsPos) 32tf - 43sj
wt amplicon start Chr3:4808670
wt amplicon end Chr3:4809254
wt amplicon size 585 bp
Locus specific primer 43sj: 5'-CAAACTTGTGAACAAAAAGCTTGA-3'
2nd locus specific primer 32tf: 5'-ATTGTATTCATTACAGGGAGCTGG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11