Genotyping and primer details


Genotyping and primer details for 675H03 - At1g32580
Line ID 675H03
Locus At1g32580 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:11784098 of line 675H03 Chr1:11784098
   
Insertion allele amplicon 8474 - 05en
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 05en: 5'-CTCTTCATCAATCTCACAACCAAA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:11784656
   
wt amplicon (over InsPos) 05fi - 05en
wt amplicon start Chr1:11783668
wt amplicon end Chr1:11784656
wt amplicon size 989 bp
Locus specific primer 05en: 5'-CTCTTCATCAATCTCACAACCAAA-3'
2nd locus specific primer 05fi: 5'-TCTTCATATGCAAATCCCCTAATC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11