Genotyping and primer details


Genotyping and primer details for 676F10 - At5g41100
Line ID 676F10
Locus At5g41100 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:16447921 of line 676F10 Chr5:16447921
   
Insertion allele amplicon 8474 - 13jk
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 13jk: 5'-ATTAGAAGAATTCCACCTGCAGAA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:16448488
   
wt amplicon (over InsPos) 24kj - 13jk
wt amplicon start Chr5:16447487
wt amplicon end Chr5:16448488
wt amplicon size 1002 bp
Locus specific primer 13jk: 5'-ATTAGAAGAATTCCACCTGCAGAA-3'
2nd locus specific primer 24kj: 5'-TTGATATAGGAGAGCTTTTTCCCA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11