Genotyping and primer details


Genotyping and primer details for 678H09 - At5g13260
Line ID 678H09
Locus At5g13260 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:4245723 of line 678H09 Chr5:4245723
   
Insertion allele amplicon 8474 - 11bn
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 11bn: 5'-CTAGAACTTTCATGCCATCGTGTA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:4245147
   
wt amplicon (over InsPos) 10cr - 11bn
wt amplicon start Chr5:4245147
wt amplicon end Chr5:4246222
wt amplicon size 1076 bp
Locus specific primer 11bn: 5'-CTAGAACTTTCATGCCATCGTGTA-3'
2nd locus specific primer 10cr: 5'-GGCTTGTAATTCTGTACCTCCTTG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11