Genotyping and primer details


Genotyping and primer details for 679H10 - At1g05500
Line ID 679H10
Locus At1g05500 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:1627430 of line 679H10 Chr1:1627430
   
Insertion allele amplicon 8474 - KU79
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer KU79: 5'-CCACGGTTCTTAGTATCTCTCTGG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:1628054
   
wt amplicon (over InsPos) 05id - KU79
wt amplicon start Chr1:1627035
wt amplicon end Chr1:1628054
wt amplicon size 1020 bp
Locus specific primer KU79: 5'-CCACGGTTCTTAGTATCTCTCTGG-3'
2nd locus specific primer 05id: 5'-GAATCAACAGGGTGTTCTGGTTAT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11