Genotyping and primer details


Genotyping and primer details for 683B06 - At1g30910
Line ID 683B06
Locus At1g30910 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:11001521 of line 683B06 Chr1:11001521
   
Insertion allele amplicon 8474 - 21cv
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 21cv: 5'-GCTGTTTCTTCATCTGGGTTTAAG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:11001131
   
wt amplicon (over InsPos) 65dy - 21cv
wt amplicon start Chr1:11001131
wt amplicon end Chr1:11001944
wt amplicon size 814 bp
Locus specific primer 21cv: 5'-GCTGTTTCTTCATCTGGGTTTAAG-3'
2nd locus specific primer 65dy: 5'-CCAAAATCTTTCAAAGGAGTCAAG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11