Genotyping and primer details


Genotyping and primer details for 685A10 - At1g13730
Line ID 685A10
Locus At1g13730 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:4711120 of line 685A10 Chr1:4711120
   
Insertion allele amplicon 8474 - MG12
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer MG12: 5'-TCTTGTATAAGTCACCATCGCAAG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:4710595
   
wt amplicon (over InsPos) 16sq - MG12
wt amplicon start Chr1:4710595
wt amplicon end Chr1:4711436
wt amplicon size 842 bp
Locus specific primer MG12: 5'-TCTTGTATAAGTCACCATCGCAAG-3'
2nd locus specific primer 16sq: 5'-CTTAGACTTAGGCTTTGCAGGAGA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11