Genotyping and primer details


Genotyping and primer details for 690B06 - At1g47900
Line ID 690B06
Locus At1g47900 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:17647550 of line 690B06 Chr1:17647550
   
Insertion allele amplicon 8474 - 57by
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 57by: 5'-CAGTATGAAAATGCACAAGTCGTT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:17647193
   
wt amplicon (over InsPos) 04dt - 57by
wt amplicon start Chr1:17647193
wt amplicon end Chr1:17648044
wt amplicon size 852 bp
Locus specific primer 57by: 5'-CAGTATGAAAATGCACAAGTCGTT-3'
2nd locus specific primer 04dt: 5'-TGAAGAAGCTGATATCAAGAGCAA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11