Genotyping and primer details


Genotyping and primer details for 690H09 - At1g69360
Line ID 690H09
Locus At1g69360 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:26073001 of line 690H09 Chr1:26073001
   
Insertion allele amplicon 8474 - 10hd
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 10hd: 5'-TTTTGTCCATTGTTACTTGCTCTG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:26072438
   
wt amplicon (over InsPos) 06ig - 10hd
wt amplicon start Chr1:26072438
wt amplicon end Chr1:26073455
wt amplicon size 1018 bp
Locus specific primer 10hd: 5'-TTTTGTCCATTGTTACTTGCTCTG-3'
2nd locus specific primer 06ig: 5'-GAAGGTAATAAAGCTGCCACTCAC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11