Genotyping and primer details


Genotyping and primer details for 691D11 - At3g46930
Line ID 691D11
Locus At3g46930 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:17287958 of line 691D11 Chr3:17287958
   
Insertion allele amplicon 8474 - 18kn
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 18kn: 5'-TTTGGACTTCTTTTATGGGAAATG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:17287467
   
wt amplicon (over InsPos) 04ls - 18kn
wt amplicon start Chr3:17287467
wt amplicon end Chr3:17288459
wt amplicon size 993 bp
Locus specific primer 18kn: 5'-TTTGGACTTCTTTTATGGGAAATG-3'
2nd locus specific primer 04ls: 5'-ATCGAGAAGATTGTGACTCCTGAT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11