Genotyping and primer details


Genotyping and primer details for 692B09 - At4g32640
Line ID 692B09
Locus At4g32640 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:15742736 of line 692B09 Chr4:15742736
   
Insertion allele amplicon 8474 - NA42
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer NA42: 5'-TTGTGTAGTTACTGCCACCAAGTC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:15742479
   
Insertion allele amplicon 8474 - NA43
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer NA43: 5'-CAGTAAACGGAGTTAATGGTGGAT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:15743423
   
wt amplicon (over InsPos) 29go - NA43
wt amplicon start Chr4:15742259
wt amplicon end Chr4:15743423
wt amplicon size 1165 bp
Locus specific primer NA43: 5'-CAGTAAACGGAGTTAATGGTGGAT-3'
2nd locus specific primer 29go: 5'-CGATAGACCCTTTTGAGATTCTTG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11