Genotyping and primer details


Genotyping and primer details for 692E08 - At1g54115
Line ID 692E08
Locus At1g54115 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:20204100 of line 692E08 Chr1:20204100
   
Insertion allele amplicon 8474 - 19nt
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 19nt: 5'-TTGGACCCATTTTGTTCTCTATTC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:20204663
   
wt amplicon (over InsPos) 34oj - 19nt
wt amplicon start Chr1:20203445
wt amplicon end Chr1:20204663
wt amplicon size 1219 bp
Locus specific primer 19nt: 5'-TTGGACCCATTTTGTTCTCTATTC-3'
2nd locus specific primer 34oj: 5'-AAGCTCGCATTGTAGCCTATTTTA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11