Genotyping and primer details


Genotyping and primer details for 697G02 - At1g11362
Line ID 697G02
Locus At1g11362 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:3824639 of line 697G02 Chr1:3824639
   
Insertion allele amplicon 8474 - 28rx
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 28rx: 5'-CGGAGGTTACAAAAAGCG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:3825096
   
wt amplicon (over InsPos) 21tl - 28rx
wt amplicon start Chr1:3824318
wt amplicon end Chr1:3825096
wt amplicon size 779 bp
Locus specific primer 28rx: 5'-CGGAGGTTACAAAAAGCG-3'
2nd locus specific primer 21tl: 5'-AGGGGCTAAAAATCCAGTCTTAAA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11