Genotyping and primer details


Genotyping and primer details for 699C04 - At1g67720
Line ID 699C04
Locus At1g67720 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:25390678 of line 699C04 Chr1:25390678
   
Insertion allele amplicon 8474 - EU41
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer EU41: 5'-AGAAGATTTTGGTCCTGAACTGAA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:25390377
   
wt amplicon (over InsPos) TM32 - EU41
wt amplicon start Chr1:25390377
wt amplicon end Chr1:25391188
wt amplicon size 812 bp
Locus specific primer EU41: 5'-AGAAGATTTTGGTCCTGAACTGAA-3'
2nd locus specific primer TM32: 5'-TGGTGCATTTGTATCATTTTGTTT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11