Genotyping and primer details


Genotyping and primer details for 700B07 - At5g45780
Line ID 700B07
Locus At5g45780 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:18569153 of line 700B07 Chr5:18569153
   
Insertion allele amplicon 8474 - EU42
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer EU42: 5'-ATTTTTGGGTTATTGTAGTGGCTG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:18569448
   
wt amplicon (over InsPos) TM33 - EU42
wt amplicon start Chr5:18568562
wt amplicon end Chr5:18569448
wt amplicon size 887 bp
Locus specific primer EU42: 5'-ATTTTTGGGTTATTGTAGTGGCTG-3'
2nd locus specific primer TM33: 5'-CACATAAGTTGCAACGGCTAATAA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11