Genotyping and primer details


Genotyping and primer details for 705E01 - At1g01710
Line ID 705E01
Locus At1g01710 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:265664 of line 705E01 Chr1:265664
   
Insertion allele amplicon 8474 - CR34
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer CR34: 5'-CTTTCAGGAGTTATCGGAACAAAG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:264790
   
wt amplicon (over InsPos) 34si - CR34
wt amplicon start Chr1:264790
wt amplicon end Chr1:266092
wt amplicon size 1303 bp
Locus specific primer CR34: 5'-CTTTCAGGAGTTATCGGAACAAAG-3'
2nd locus specific primer 34si: 5'-TCTTTTTATTCGTCGCCTTGTACT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11