Genotyping and primer details


Genotyping and primer details for 713E03 - At2g35860
Line ID 713E03
Locus At2g35860 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:15061062 of line 713E03 Chr2:15061062
   
Insertion allele amplicon 8474 - 25vz
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 25vz: 5'-TTCATCCACACTCTCTTGCATTAC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:15060636
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11