Genotyping and primer details


Genotyping and primer details for 714H09 - At1g66260
Line ID 714H09
Locus At1g66260 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:24696759 of line 714H09 Chr1:24696759
   
Insertion allele amplicon 8474 - 53sm
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 53sm: 5'-TTTCCCTACGTTTCATTTGGTATC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:24697097
   
wt amplicon (over InsPos) 31tc - 53sm
wt amplicon start Chr1:24696140
wt amplicon end Chr1:24697097
wt amplicon size 958 bp
Locus specific primer 53sm: 5'-TTTCCCTACGTTTCATTTGGTATC-3'
2nd locus specific primer 31tc: 5'-ACATGAATGACAACGAGATCAGAC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11