Genotyping and primer details


Genotyping and primer details for 717D10 - At4g03000
Line ID 717D10
Locus At4g03000 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:1324696 of line 717D10 Chr4:1324696
   
Insertion allele amplicon 8474 - 54sm
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 54sm: 5'-CGTGTTTTGTTATTTGGTTGAATG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:1324308
   
wt amplicon (over InsPos) 08tl - 54sm
wt amplicon start Chr4:1324308
wt amplicon end Chr4:1325228
wt amplicon size 921 bp
Locus specific primer 54sm: 5'-CGTGTTTTGTTATTTGGTTGAATG-3'
2nd locus specific primer 08tl: 5'-AGCTTTCAAAACATTCAAGTCACA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11