Genotyping and primer details


Genotyping and primer details for 717E09 - At2g24260
Line ID 717E09
Locus At2g24260 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:10320498 of line 717E09 Chr2:10320498
   
Insertion allele amplicon 8474 - 30uv
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 30uv: 5'-TTGTTTTTCATACGTACAAGGGGT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:10320782
   
wt amplicon (over InsPos) 35vx - 30uv
wt amplicon start Chr2:10319949
wt amplicon end Chr2:10320782
wt amplicon size 834 bp
Locus specific primer 30uv: 5'-TTGTTTTTCATACGTACAAGGGGT-3'
2nd locus specific primer 35vx: 5'-CACTTGATGTTCCGTCATTGTAAC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11