Genotyping and primer details


Genotyping and primer details for 718A03 - At1g70480
Line ID 718A03
Locus At1g70480 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:26563529 of line 718A03 Chr1:26563529
   
Insertion allele amplicon 8474 - 35cp
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 35cp: 5'-GTGAATGTTGGTTTACCTCCTGAT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:26562809
   
wt amplicon (over InsPos) 19dw - 35cp
wt amplicon start Chr1:26562809
wt amplicon end Chr1:26563689
wt amplicon size 881 bp
Locus specific primer 35cp: 5'-GTGAATGTTGGTTTACCTCCTGAT-3'
2nd locus specific primer 19dw: 5'-TATGTTCACATATTGCTGGGTGAT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11