Genotyping and primer details


Genotyping and primer details for 719D11 - At5g04060
Line ID 719D11
Locus At5g04060 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:1100207 of line 719D11 Chr5:1100207
   
Insertion allele amplicon 8474 - GE48
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer GE48: 5'-AACACGACATCTTGAACAGTGAAC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:1100506
   
wt amplicon (over InsPos) 13gl - GE48
wt amplicon start Chr5:1099713
wt amplicon end Chr5:1100506
wt amplicon size 794 bp
Locus specific primer GE48: 5'-AACACGACATCTTGAACAGTGAAC-3'
2nd locus specific primer 13gl: 5'-ACAAAATAGAGCTCACTTACCGGA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11