Genotyping and primer details


Genotyping and primer details for 721C11 - At3g60030
Line ID 721C11
Locus At3g60030 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:22168950 of line 721C11 Chr3:22168950
   
Insertion allele amplicon 8474 - PG13
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer PG13: 5'-ATGGAAGCTAGAATTGAAGGTGAA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:22169410
   
wt amplicon (over InsPos) 06fz - PG13
wt amplicon start Chr3:22168375
wt amplicon end Chr3:22169410
wt amplicon size 1036 bp
Locus specific primer PG13: 5'-ATGGAAGCTAGAATTGAAGGTGAA-3'
2nd locus specific primer 06fz: 5'-CAATATTTTGAGAAGCCTGGAGTC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11